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Table 1 Strains and plasmids used in this study

From: Engineering Escherichia coli for high-yield geraniol production with biotransformation of geranyl acetate to geraniol under fed-batch culture

Name Relevant characteristics References
primersa
 GES-rbs-F GGAAGATCTAGGAGGTAAAAAATATGTCTTGCGCTCGTATCACCG This study
 GES-R CCGCTCGAGTTACTGGGTGAAGAACAGAGCG This study
 Aes-F-NcoI CCCATGGCTATGAAGCCGGAAAACAAACT This study
 Aes-R-EcoRI GGAATTCCTAAAGCTGAGCGGTAAAGAACTG This study
Strains
 BL21(DE3) F omp T, hsdSB (r B m B ), gal, dcm me131, λ(DE3) Invitrogen
 LWG6 E.coli BL21(DE3)/pLWG6, pYJM14 This study
 LWG10 E.coli BL21(DE3)/p YJM26, pYJM14 This study
 LWG11 E.coli BL21(DE3)/p LWG11 This study
Plasmids
 pET-30a F1 (pBR322), Kan Novagen
 pGH/GES pGH carrying GES from O. basilicum This study
 pLWG 6 pACYCDuet-1 carrying mvaE and mvaS from E. faecalis, GPPS2 from A. grandis,GES from O. basilicum, Cm This study
 pLWG11 pET-30a carrying aes from E. coli, Kan This study
 pYJM26 pACYCDuet-1 carrying mvaE and mvaS from E. faecalis, GPPS2 from A. grandis, Cm [27]
 pYJM14 pTrcHis2B carrying ERG12, ERG8, ERG19 and IDI1 from S.cerevisiae, Amp [27]
  1. a Restriction sites are underlined