Table 1 Strains and plasmids used in this study
Name | Relevant characteristics | References |
|---|---|---|
primersa | ||
 GES-rbs-F | GGAAGATCTAGGAGGTAAAAAATATGTCTTGCGCTCGTATCACCG | This study |
 GES-R | CCGCTCGAGTTACTGGGTGAAGAACAGAGCG | This study |
 Aes-F-NcoI | CCCATGGCTATGAAGCCGGAAAACAAACT | This study |
 Aes-R-EcoRI | GGAATTCCTAAAGCTGAGCGGTAAAGAACTG | This study |
Strains | ||
 BL21(DE3) | F− omp T, hsdSB (r −B m −B ), gal, dcm me131, λ(DE3) | Invitrogen |
 LWG6 | E.coli BL21(DE3)/pLWG6, pYJM14 | This study |
 LWG10 | E.coli BL21(DE3)/p YJM26, pYJM14 | This study |
 LWG11 | E.coli BL21(DE3)/p LWG11 | This study |
Plasmids | ||
 pET-30a | F1 (pBR322), Kan | Novagen |
 pGH/GES | pGH carrying GES from O. basilicum | This study |
 pLWG 6 | pACYCDuet-1 carrying mvaE and mvaS from E. faecalis, GPPS2 from A. grandis,GES from O. basilicum, Cm | This study |
 pLWG11 | pET-30a carrying aes from E. coli, Kan | This study |
 pYJM26 | pACYCDuet-1 carrying mvaE and mvaS from E. faecalis, GPPS2 from A. grandis, Cm | [27] |
 pYJM14 | pTrcHis2B carrying ERG12, ERG8, ERG19 and IDI1 from S.cerevisiae, Amp | [27] |