Systematic engineering of the central metabolism in Escherichia coli for effective production of n-butanol

Table 1 Summary of the fermentation kinetics for main engineered strains

Strain	Y_B (g/L)	P_B (g/L/h)	Y_B/G (g/g)	NADH (μmol/g cell)	Specific enzyme activity (U/mg protein)
Strain	Y_B (g/L)	P_B (g/L/h)	Y_B/G (g/g)	NADH (μmol/g cell)	PDH	Zwf	Pgl	GltA
BuT-8	2.7^a	0.11^a	0.14^a	42.2	0.7	ND	ND	ND
BuT-9	4.3	0.18	0.22	60.9	1.6	6.1	ND	ND
BuT-10	4.9	0.20	0.25	75.8	18.3	0.5	ND	ND
BuT-12	5.4	0.23	0.27	82.6	ND	ND	6.2	2.2
BuT-14	6.1	0.21	0.31	96.1	ND	ND	ND	1.5

^aThe kinetic data were drawn from the previous report [19]. The development course of producer strains for the n-butanol production was shown in Additional file 1: Fig. S1. U μmole/min, Y _B the n-butanol titer, ND not determined

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