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Table 2 Carbon recovery of fermentation products for engineered strains during the oxygen-limited growth on glucose

From: Systematic engineering of the central metabolism in Escherichia coli for effective production of n-butanol

Strain

Pyruvate

Succinate

Ethanol

Lactate

Acetate

Butyrate

Butanol

Total (%)

BuT-8

20.7

3.5

7.8

2.7

1.5

4.1

21.9

62.2

BuT-9

2.6

2.1

5.2

1.8

1.2

2.7

34.8

50.4

BuT-10

0.01

1.1

5.2

1.5

0.8

2.0

39.6

50.2

BuT-12

Nil

0.8

5.2

1.4

0.7

1.4

43.7

53.2

BuT-14

Nil

0.6

2.6

1.3

0.5

1.4

49.4

55.8

  1. Carbon recovery was calculated as the molar percent of carbon in products per carbon in consumed glucose. Nil, carbon recovery less than 0.01