Fig. 2

Starch and oil hyper-accumulate in std1 mutant following N deprivation. a Starch content in cells grown photoautotrophically under low light (LL, 35 µmol photons m⁻² s⁻¹) supplemented with 2 % CO₂. b Starch content in cells grown photoautotrophically under medium light (100 µmol photons m⁻² s⁻¹) supplemented with 2 % CO₂. c Starch content in cells grown mixotrophically (i.e., TAP) under medium light (100 µmol photons m⁻² s⁻¹). d TAG content in cells grown photoautotrophically under medium light (100 µmol photons m⁻² s⁻¹) supplemented with 2 % CO₂. e TAG content in cells grown mixotrophically (i.e., TAP media) under medium light (100 µmol photons m⁻² s⁻¹). f Nile red fluorescence (lower panel) and bright field microscopy (upper panel) of indicated cells under photoautotrophic N deprivation. Scale bar 10 µm. MM minimal medium. Data are means ± SD (n = 3). Cell counts, cellular volumes, and chlorophyll content measurements related to these experiments are shown on Additional file 1: Figure S4