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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Hyper-accumulation of starch and oil in a Chlamydomonas mutant affected in a plant-specific DYRK kinase

Fig. 4

Biomass productivity of WT and std1 Chlamydomonas cells measured in photobioreactors operated as turbidostats during photoautotrophic N deprivation. Cells were grown under constant illumination (125 µmol photons m−2 s−1) in the presence of 2 % CO2 enriched air. Cell density was measured using an absorption probe and maintained at a constant level by injection of fresh medium. Due to the aggregation phenotype of std1, OD880nm was regulated at different values for WT (OD880nm = 0. 4) and std1 (OD880nm = 0. 3) to reach similar biomass concentrations (0. 15 g dry weight L−1). After a 48-h stabilization period in the presence of MM, the dilution medium was replaced by MM-N (t 0). a Cumulated amounts of fresh medium were added to maintain the culture at a constant biomass concentration. Measurements of ammonium concentration (dotted lines) in the culture medium showed complete exhaustion after 45 h. The 100 % value corresponds to 7.5 mM NH4 +, which is the ammonium concentration of the minimal medium. Shown are mean ± SD (n = 3). b Biomass productivity (g dry weight L−1 d−1) was determined from dilution rates and biomass measurements at t 0, and 48 and 72 h after N-removal. Shown are means ± SD (n = 3)

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