From: Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae
Variant | Mutation | Amino acid | Substitution | Relative activity |
---|---|---|---|---|
v3 | 923A > G | 305 | Tyr → Cys | 1.68 ± 0.02 |
1557A > T | 516 | – | 1.05 ± 0.01 | |
1934A > T | 642 | Glu → Val | 0.80 ± 0.03 | |
v4a | 65T > C | 22 | Val → Ala | 1.57 ± 0.06 |
174A > T | 55 | – | ||
465C > T | 152 | – | ||
1707C > T | 566 | – | 0.98 ± 0.03 | |
v5 | 428A > T | 140 | Gln → Leu | 1.56 ± 0.05 |
2067A > T | 686 | – | 1.03 ± 0.02 | |
2103T > A | 698 | – | 1.07 ± 0.03 | |
v6 | 917T > C | 303 | Val → Ala | 1.09 ± 0.04 |
1707C > T | 566 | – | 0.98 ± 0.03 | |
1818T > C | 603 | – | 1.05 ± 0.04 | |
v7a | 65T > A | 22 | Val → Asp | 2.03 ± 0.08 |
297T > C | 96 | – | ||
643A > G | 212 | Ile-Val | ||
892T > C | 295 | – | ||
1814A > G | 602 | Lys → Arg | ||
2019T > C | 670 | – | ||
2079T > A | 690 | – | ||
v8a | 25G > A | 9 | Ala → Thr | |
60T > A | 20 | – | ||
61C > A | 21 | Pro → Thr | 1.77 ± 0.02 | |
93T > C | 28 | – | ||
462T > C | 151 | – | ||
981C > T | 324 | – | ||
1158C > T | 383 | – | ||
1953C > T | 648 | – | ||
2389A > C | 794 | Lys → Gln | ||
v10 | 1180T > C | 391 | – | 0.90 ± 0.02 |
1448C > T | 480 | Ala → Val | Not determined | |
1506T > A | 499 | – | 0.88 ± 0.05 | |
v11a | 61C > T | 21 | Pro → Ser | 1.93 ± 0.02 |
366T > C | 119 | – | ||
954C > T | 315 | – | ||
1773C > T | 588 | – | ||
v16 | 681T > C | 224 | – | 0.86 ± 0.01 |
1489A > C | 494 | Lys → Gln | 1.17 ± 0.06 | |
1678A > G | 557 | Asn → Asp | 1.19 ± 0.06 | |
v18a | 65T > A | 22 | Val → Asp | 2.03 ± 0.08 |
1983C > T | 658 | – | ||
v19a | 65T > C | 22 | Val → Ala | 1.57 ± 0.06 |
219G > A | 70 | – | ||
1275A > G | 422 | – | ||
2367T > G | 786 | – | ||
2441C > T | 811 | Thr → Met | ||
v20 | 428A > T | 140 | Gln → Leu | 1.56 ± 0.05 |
1925A > T | 639 | Lys → Met | 0.73 ± 0.04 | |
2349T > A | 780 | – | 0.94 ± 0.01 |