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Table 2 Kinetic parameters of BGL1 and evolved variants for synthetic substrate

From: Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae

  app K m (mM) app V max (μmole L−1 min−1) app K i glucose (mM) app K i pNPG (mM)
BGL1 0.82 ± 0.12 118.5 ± 10.6 2.98 ± 0.46
V22D 0.93 ± 0.23 210.5 ± 31.6 2.98 ± 0.75
Q140L 1.09 ± 0.06 249.6 ± 9.2 3.41 ± 0.21
Y305C 0.77 ± 0.03 149.7 ± 1.7 1.81 ± 0.08
A480V 0.98 ± 0.25 217.7 ± 34.3 3.26 ± 0.87
K494Q 0.89 ± 0.12 141.5 ± 11.1 3.06 ± 0.41
N557D 0.88 ± 0.09 186.2 ± 11.0 3.16 ± 0.32