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Table 2 Kinetic parameters of BGL1 and evolved variants for synthetic substrate

From: Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae

 

app K m (mM)

app V max (μmole L−1 min−1)

app K i glucose (mM)

app K i pNPG (mM)

BGL1

0.82 ± 0.12

118.5 ± 10.6

2.98 ± 0.46

V22D

0.93 ± 0.23

210.5 ± 31.6

2.98 ± 0.75

Q140L

1.09 ± 0.06

249.6 ± 9.2

3.41 ± 0.21

Y305C

0.77 ± 0.03

149.7 ± 1.7

1.81 ± 0.08

A480V

0.98 ± 0.25

217.7 ± 34.3

3.26 ± 0.87

K494Q

0.89 ± 0.12

141.5 ± 11.1

3.06 ± 0.41

N557D

0.88 ± 0.09

186.2 ± 11.0

3.16 ± 0.32