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Table 3 Kinetic parameters of Y305C substituted variants for synthetic substrate

From: Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae

 

app K m (mM)

app V max (μmole mg−1 min−1)

app K i glucose (mM)

Y305C

0.93 ± 0.04

71.0 ± 0.9

1.92 ± 0.10

V22D/Y305C

1.06 ± 0.04

127.4 ± 1.3

2.22 ± 0.09

Q140L/Y305C

1.32 ± 0.05

111.1 ± 1.4

3.18 ± 0.15

Y305C/A480V

1.06 ± 0.05

124.3 ± 1.8

2.22 ± 0.12

DLCV

1.69 ± 0.08

221.3 ± 3.6

3.37 ± 0.18