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Table 3 Kinetic parameters of Y305C substituted variants for synthetic substrate

From: Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae

  app K m (mM) app V max (μmole mg−1 min−1) app K i glucose (mM)
Y305C 0.93 ± 0.04 71.0 ± 0.9 1.92 ± 0.10
V22D/Y305C 1.06 ± 0.04 127.4 ± 1.3 2.22 ± 0.09
Q140L/Y305C 1.32 ± 0.05 111.1 ± 1.4 3.18 ± 0.15
Y305C/A480V 1.06 ± 0.05 124.3 ± 1.8 2.22 ± 0.12
DLCV 1.69 ± 0.08 221.3 ± 3.6 3.37 ± 0.18