Skip to main content

Table 4 Kinetic parameters of Tyr305 variants for synthetic substrate

From: Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae

  app K m (mM) app V max (μmole L−1 min−1) app K i pNPG (mM)
Y305F 0.46 ± 0.04 126.5 ± 5.4 4.78 ± 0.44
Y305Wa NA NA NA
Y305G 1.16 ± 0.06 239.7 ± 3.9
Y305A 1.55 ± 0.06 238.2 ± 4.1
Y305 V 1.14 ± 0.08 233.9 ± 5.6
  1. aInactive under all tested conditions