Table 5 Kinetic parameters of BGLs for natural substrate
From: Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae
app K m (mM) | app V max (μmole L−1 min−1) | app K i cellobiose (mM) | |
|---|---|---|---|
BGL1 | 1.2 ± 0.1 | 61.9 ± 2.5 | 32.4 ± 4.5 |
Y305C | 5.3 ± 0.6 | 106.4 ± 3.4 | – |
Y305G | 5.6 ± 0.7 | 122.4 ± 4.3 | – |
DLCV | 3.6 ± 0.3 | 207.5 ± 4.3 | – |