Key enzymes catalyzing glycerol to 1,3-propanediol

Table 3 The properties of PDORs from different microbial sources

Strains	Relative molecular mass (kD)	Subunit relative molecular mass (kD)	Specific activity (U/mg)	Km (mmol/L)	Optimal temperature ( °C)	Optimal pH	Metal ions with the enzyme activity
L. brevis	350	41–46	7.28	300	37	6.6	Fe²⁺, Mn²⁺: the enzyme activation factors
K. pneumoniae	336	42	37	18	30	9.5	Fe²⁺, Mn²⁺: the enzyme activation factors
C. freundii	347	43.4	11	140	37	7.7	Fe²⁺ or Mn²⁺ can make its recovery activities
E. agglomerans	355 ± 5	38.5 ± 0.5	3.42	13.7	37	7.8	Mn²⁺: the enzyme activation factors
K. pneumoniae DSM2026	387	41.5	9.85	–	55	10.0	Fe²⁺, Na⁺, NH⁴⁺, and Mn²⁺: auxo-action on the enzyme activity
C. butyricum	384.2 ± 31.1	42	4.51	0.17	37	9.07	Li²⁺, Mn²⁺, and Na⁺: the enzyme activation factors

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