Fig. 1

Investigation of the binding specificity of the expansin dockerin with various cohesins. The specific binding of the CclEXL1 dockerin was determined by affinity-based ELISA. Microtitter plates of 96 wells were coated with CclEXL1, and (a) five CBM-Coh fusion proteins from C. clariflavum (Cohesins A1, A5, A8, D3, and G) or (b) eight CBM-Coh fusion proteins from different bacterial species (C. clariflavum, C. cellulolyticum, C. thermocellum, B. cellulosolvens, A. cellulolyticus, R. flavefaciens, and A. fulgidus) were added to the wells in increasing concentrations. CBM, carbohydrate-binding module; Coh, cohesin