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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Cellulosomal expansin: functionality and incorporation into the complex

Fig. 4

Preincubation of filter paper strips with CclEXL1 enhances enzymatic degradation. Filter paper strips were incubated for 1 h at 55 °C with either 600 µg/mL CclEXL1 or acetate buffer (pH 5.5) (a, b). For (c, d), no preincubation step was performed, and CclEXL1 was added to the reaction together with the enzymes and cellulosome. After incubation, the strips were supplemented with either 0.5 µM of two recombinant C. clariflavum cellulases, GH48 and GH9, or 25 µg/mL of the C. clariflavum MCCI cellulosome fraction, and hydrolysis was carried out for 24 h at 55 °C. The released reducing sugars in the solutions (a, c) and the reducing sugars on the filter paper (b, d) were measured. Standard deviations are indicated, where three asterisks (***) indicate p < 0.001, two asterisks (**) indicate p < 0.01, and a single asterisk indicates p < 0.05 (two-tailed t test)

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