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Table 10 Identified fungal cell wall degradation/modifying enzymes and spectrum counts in different carbon sources by wild-type 2HH and mutant S1M29 of Penicillium echinulatum at 96 h during submerged cultivation

From: Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass

Accession number Identified proteins CAZyme Organism MW (kDa) Secretionb Spectrum counta
2HH S1M29
Glu Gly SCB Cel Glu Gly SCB Cel
g7097 Chitin glucanosyltransferase GH16 Penicillium oxalicum 39 Y 41 11 12 13 32 0 9 10
g1080 Chitinase1 GH18 Penicillium oxalicum 49 Y 1 25 0 1 0 105 0 1
g8496 Chitinase 1, class 5 CBM50 Grosmannia clavigera 37 Y 16 0 0 0 0 0 0 0
g1258 Exo-β-1,3-glucanase GH17 Penicillium oxalicum 47 Y 72 5 4 4 15 10 1 3
g4052 β-1,3-glucanosyltransferase GH17 Penicillium oxalicum 33 Y 9 8 3 15 4 9 1 4
g284 β-N-acetylhexosaminidase GH3 Penicillium oxalicum 38 Y 29 19 7 3 0 10 0 0
g244 β-1,3-glucanosyltransglycosylase GH72 Penicillium oxalicum 49 Y 0 9 7 6 0 11 1 6
g3495 Chitosanase GH75 Penicillium oxalicum 37 Y 30 12 3 3 1 2 0 2
g5309 Exo-β-1,3-glucanase GH5 Penicillium oxalicum 45 Y 0 3 5 8 0 0 0 0
g6946 Chitin glucanosyltransferase GH16 (CBM 18) Penicillium oxalicum 47 Y 5 6 5 2 0 3 1 2
g980 β-1,6-N-acetyl glucosaminidase GH20 Penicillium oxalicum 67 Y 0 4 4 2 0 4 0 1
g2799 β-1,3-glucanosyl transglycosylase GH72 Penicillium oxalicum 53 Y 0 0 2 1 0 0 0 1
g1191 β-1,3-glucanosyl transglycosylase GH72 (CBM 43) Penicillium oxalicum 57 Y 2 0 1 0 0 0 0 1
g3950 Exo-β-1,3-glucanase GH5 Penicillium oxalicum 46 Y 0 1 0 0 0 3 0 0
g6368 Chitinase GH18 Penicillium oxalicum 47 Y 0 3 0 3 0 0 0 0
g4641 β-1,3-glucanase GH17 Penicillium oxalicum 68 N 3 3 0 0 0 0 0 0
g5953 β-1,6-glucanase GH30 Penicillium oxalicum 52 Y 0 0 0 0 0 0 1 0
g8051 α-1,3-glucanase GH71 (CBM24) Penicillium oxalicum 114 Y 7 0 0 2 0 0 0 5
  1. aSecretomic analysis based on spectral counting. A quantitative analysis was conducted for samples grown on cellulose or SCB (mean of triplicates), while a semiquantitative analysis for samples grown on glucose or glycerol (one replicate) was performed. The complete protein reports are given in Additional file 3
  2. bThe secretion of each protein was verified by the softwares SignalP, SecretomeP and YLoc. When at least two of three softwares give a positive result the protein was considered secreted