Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass

Table 10 Identified fungal cell wall degradation/modifying enzymes and spectrum counts in different carbon sources by wild-type 2HH and mutant S1M29 of Penicillium echinulatum at 96 h during submerged cultivation

Accession number	Identified proteins	CAZyme	Organism	MW (kDa)	Secretion^b	Spectrum count^a
						2HH				S1M29
						Glu	Gly	SCB	Cel	Glu	Gly	SCB	Cel
g7097	Chitin glucanosyltransferase	GH16	Penicillium oxalicum	39	Y	41	11	12	13	32	0	9	10
g1080	Chitinase1	GH18	Penicillium oxalicum	49	Y	1	25	0	1	0	105	0	1
g8496	Chitinase 1, class 5	CBM50	Grosmannia clavigera	37	Y	16	0	0	0	0	0	0	0
g1258	Exo-β-1,3-glucanase	GH17	Penicillium oxalicum	47	Y	72	5	4	4	15	10	1	3
g4052	β-1,3-glucanosyltransferase	GH17	Penicillium oxalicum	33	Y	9	8	3	15	4	9	1	4
g284	β-N-acetylhexosaminidase	GH3	Penicillium oxalicum	38	Y	29	19	7	3	0	10	0	0
g244	β-1,3-glucanosyltransglycosylase	GH72	Penicillium oxalicum	49	Y	0	9	7	6	0	11	1	6
g3495	Chitosanase	GH75	Penicillium oxalicum	37	Y	30	12	3	3	1	2	0	2
g5309	Exo-β-1,3-glucanase	GH5	Penicillium oxalicum	45	Y	0	3	5	8	0	0	0	0
g6946	Chitin glucanosyltransferase	GH16 (CBM 18)	Penicillium oxalicum	47	Y	5	6	5	2	0	3	1	2
g980	β-1,6-N-acetyl glucosaminidase	GH20	Penicillium oxalicum	67	Y	0	4	4	2	0	4	0	1
g2799	β-1,3-glucanosyl transglycosylase	GH72	Penicillium oxalicum	53	Y	0	0	2	1	0	0	0	1
g1191	β-1,3-glucanosyl transglycosylase	GH72 (CBM 43)	Penicillium oxalicum	57	Y	2	0	1	0	0	0	0	1
g3950	Exo-β-1,3-glucanase	GH5	Penicillium oxalicum	46	Y	0	1	0	0	0	3	0	0
g6368	Chitinase	GH18	Penicillium oxalicum	47	Y	0	3	0	3	0	0	0	0
g4641	β-1,3-glucanase	GH17	Penicillium oxalicum	68	N	3	3	0	0	0	0	0	0
g5953	β-1,6-glucanase	GH30	Penicillium oxalicum	52	Y	0	0	0	0	0	0	1	0
g8051	α-1,3-glucanase	GH71 (CBM24)	Penicillium oxalicum	114	Y	7	0	0	2	0	0	0	5

^aSecretomic analysis based on spectral counting. A quantitative analysis was conducted for samples grown on cellulose or SCB (mean of triplicates), while a semiquantitative analysis for samples grown on glucose or glycerol (one replicate) was performed. The complete protein reports are given in Additional file 3
^bThe secretion of each protein was verified by the softwares SignalP, SecretomeP and YLoc. When at least two of three softwares give a positive result the protein was considered secreted

ISSN: 2731-3654