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Table 2 Identified cellulose-degradation/modifying enzymes and spectrum counts in different carbon sources by wild-type 2HH and mutant S1M29 of Penicillium echinulatum at 96 h during submerged cultivation

From: Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass

Accession number

Identified proteins

CAZyme

Organism

MW (kDa)

Secretionb

Spectral counta

2HH

S1M29

Glu

Gly

SCB

Cel

Glu

Gly

SCB

Cel

g2773

Cellobiohydrolase I

GH7 (CBM1)

Penicillium decumbens

56

Y

0

0

81

107

0

2

202

80

g8068

Cellobiohydrolase II

GH6 (CBM1)

Penicillium decumbens

49

Y

0

0

16

4

0

0

46

62

g8473

Endoglucanase 1

GH5 (CBM1)

Penicillium echinulatum

44

Y

0

0

19

27

0

1

75

59

g5809

Endoglucanase Cel5C

GH5 (CBM1, CBM46)

Penicillium decumbens

69

Y

0

1

28

30

0

1

56

58

g2788

Endoglucanase I

GH7 (CBM1)

Penicillium decumbens

50

Y

0

0

5

4

0

0

14

10

g2291

Endoglucanase II

GH5 (CBM1)

Penicillium decumbens

44

Y

0

0

4

4

0

0

13

7

g2659

Endo-β-1,4-glucanase

GH5 (CBM1)

Penicillium oxalicum

52

Y

27

0

0

3

49

1

0

6

g3996

Endo-β-1,4-glucanase

GH12

Penicillium oxalicum

26

Y

0

0

9

10

0

0

12

8

g7274

Endo-β-1,4-glucanase

GH5

Penicillium oxalicum

55

N

0

0

5

1

0

0

0

0

g6766

β-glucosidase I

GH3

Penicillium decumbens

93

Y

0

7

2

1

1

17

2

0

g8479

Related to β-glucosidase

GH132

Colletotrichum gloeosporioides

46

Y

0

2

0

22

4

0

0

0

g3303

Cellulose monooxygenase Cel61A

AA9

Penicillium oxalicum

26

Y

0

0

3

0

0

0

20

11

g8342

Cellulose monooxygenase

AA9

Penicillium oxalicum

45

Y

0

0

3

0

0

0

0

0

g5167

Swollenin

CBM1, CBM63

Penicillium oxalicum

52

Y

0

0

1

4

0

0

9

6

g1074

GMC oxidoreductase

AA3

Neosartorya fischeri

70

Y

0

0

0

0

0

1

0

0

g3947

GMC oxidoreductase

AA3

Penicillium roqueforti

67

N

0

4

0

0

0

0

0

0

  1. aSecretomic analysis based on spectral counting. A quantitative analysis was conducted for samples grown on cellulose or SCB (mean of triplicates), while a semiquantitative analysis for samples grown on glucose or glycerol (one replicate) was performed. The complete protein reports are given in Additional file 3
  2. bThe secretion of each protein was verified by the softwares SignalP, SecretomeP, and YLoc. When at least two of three softwares give a positive result, the protein was considered secreted