Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass

Table 4 Identified pectin-degradation enzymes and spectrum counts in different carbon sources by wild-type 2HH and mutant S1M29 of Penicillium echinulatum at 96 h during submerged cultivation

Accession number	Identified proteins	CAZyme	Organism	MW (kDa)	Secretion^b	Spectrum count^a
						2HH				S1M29
						Glu	Gly	SCB	Cel	Glu	Gly	SCB	Cel
g4880	Pectin lyase	PL1	Penicillium oxalicum	40	Y	0	0	0	1	12	0	0	0
g3609	Exo-α-l-1,5-arabinanase	GH93	Penicillium oxalicum	44	Y	0	0	0	2	0	0	0	0

^aSecretomic analysis based on spectral counting. A quantitative analysis was conducted for samples grown on cellulose or SCB (mean of triplicates), while a semiquantitative analysis for samples grown on glucose or glycerol (one replicate) was performed. The complete protein reports are given in Additional file 3
^bThe secretion of each protein was verified by the softwares SignalP, SecretomeP and YLoc. When at least two of three softwares give a positive result the protein was considered secreted

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