Expression of GPAT in P. tricornutum.
a Schematic map of the PtGPAT expression cassette in pHY-PtGPAT. b Expression of GPAT transcripts measured by qPCR; β-actin was used as internal reference gene. Significant difference between control and treatment groups is indicated at P < 0.05 (*) or P < 0.01 (**) level. Each value represents mean ± SD (n = 3). c GPAT protein expression detected by Western blotting with an anti-c-Myc antibody; GAPDH was used as internal reference protein