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Table 6 Description of quality traits measured on stem material of 23 maize silage hybrids diverging in cell wall digestibility

From: Maize feedstocks with improved digestibility reduce the costs and environmental impacts of biomass pretreatment and saccharification

Trait Unit Description
CW g kg−1 DM Stem cell wall content; determined as neutral detergent fiber (NDF)
Cel g kg−1 DM Stem cellulose content; determined as the difference between acid detergent fiber (ADF) and acid insoluble lignin (ADL)
Lig g kg−1 DM Stem acid insoluble lignin content; determined as ADL
Cel/CW g kg−1 CW Stem cellulose content relative to cell wall content (CW)
Hem/CW g kg−1 CW Stem hemicellulose content relative to cell wall content (CW)
Lig/CW g kg−1 CW Stem acid insoluble lignin content relative to cell wall content (CW)
pCa I g kg−1 CW Esterified p-coumaric acid released after alkaline hydrolysis of the cell wall at 25 °C
pCa II g kg−1 CW Total p-coumaric acid released after alkaline hydrolysis of the cell wall at 170 °C
FA I g kg−1 CW Esterified ferulic acid released after alkaline hydrolysis of the cell wall at 25 °C
FA II g kg−1 CW Total ferulic acid released after alkaline hydrolysis of the cell wall at 170 °C
Di-FA I g kg−1 CW Esterified di-ferulic acid released after alkaline hydrolysis of the cell wall at 25 °C
Di-FA II g kg−1 CW Total di-ferulic acid released after alkaline hydrolysis of the cell wall at 170 °C
DHS % Degree of hemicellulose substitution, expressed as the ratio of cell wall arabinose to cell wall xylose (Ara/Xyl)
H g kg−1 CW H lignin content estimated as 4-p-hydroxybenzaldehyde released following nitrobenzene oxidation of the cell wall at 170 °C
S g kg−1 CW S lignin content estimated as syringylaldehyde released following nitrobenzene oxidation of the cell wall at 170 °C
G g kg−1 CW G lignin content estimated as vanillin released following nitrobenzene oxidation of the cell wall at 170 °C
Glu-Sol g kg−1 DM Amount of glucose released from 1 g of dry biomass into pretreatment liquors following thermochemical pretreatment
Glu-Rel g kg−1 DM Amount of glucose released from 1 g of dry biomass after pretreatment and enzymatic saccharification
Glu-Con % CW glucose Percentage of total cell wall glucose released after pretreatment and enzymatic saccharification
CWD % NDF In vitro ruminal cell wall digestibility; determined as the difference in NDF content before and after sample incubation in rumen liquor for 48 h relative to NDF content prior to incubation