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Table 6 Description of quality traits measured on stem material of 23 maize silage hybrids diverging in cell wall digestibility

From: Maize feedstocks with improved digestibility reduce the costs and environmental impacts of biomass pretreatment and saccharification

Trait

Unit

Description

CW

g kg−1 DM

Stem cell wall content; determined as neutral detergent fiber (NDF)

Cel

g kg−1 DM

Stem cellulose content; determined as the difference between acid detergent fiber (ADF) and acid insoluble lignin (ADL)

Lig

g kg−1 DM

Stem acid insoluble lignin content; determined as ADL

Cel/CW

g kg−1 CW

Stem cellulose content relative to cell wall content (CW)

Hem/CW

g kg−1 CW

Stem hemicellulose content relative to cell wall content (CW)

Lig/CW

g kg−1 CW

Stem acid insoluble lignin content relative to cell wall content (CW)

pCa I

g kg−1 CW

Esterified p-coumaric acid released after alkaline hydrolysis of the cell wall at 25 °C

pCa II

g kg−1 CW

Total p-coumaric acid released after alkaline hydrolysis of the cell wall at 170 °C

FA I

g kg−1 CW

Esterified ferulic acid released after alkaline hydrolysis of the cell wall at 25 °C

FA II

g kg−1 CW

Total ferulic acid released after alkaline hydrolysis of the cell wall at 170 °C

Di-FA I

g kg−1 CW

Esterified di-ferulic acid released after alkaline hydrolysis of the cell wall at 25 °C

Di-FA II

g kg−1 CW

Total di-ferulic acid released after alkaline hydrolysis of the cell wall at 170 °C

DHS

%

Degree of hemicellulose substitution, expressed as the ratio of cell wall arabinose to cell wall xylose (Ara/Xyl)

H

g kg−1 CW

H lignin content estimated as 4-p-hydroxybenzaldehyde released following nitrobenzene oxidation of the cell wall at 170 °C

S

g kg−1 CW

S lignin content estimated as syringylaldehyde released following nitrobenzene oxidation of the cell wall at 170 °C

G

g kg−1 CW

G lignin content estimated as vanillin released following nitrobenzene oxidation of the cell wall at 170 °C

Glu-Sol

g kg−1 DM

Amount of glucose released from 1 g of dry biomass into pretreatment liquors following thermochemical pretreatment

Glu-Rel

g kg−1 DM

Amount of glucose released from 1 g of dry biomass after pretreatment and enzymatic saccharification

Glu-Con

% CW glucose

Percentage of total cell wall glucose released after pretreatment and enzymatic saccharification

CWD

% NDF

In vitro ruminal cell wall digestibility; determined as the difference in NDF content before and after sample incubation in rumen liquor for 48 h relative to NDF content prior to incubation