Table 1 Response of two-level full factorial design for screening of variables for CMCase and protease production
Run | Sucrose | Peptone | NaH2PO4 | pH | Moisture | CMCase activity (U/g) | Protease activity (U/g) |
|---|---|---|---|---|---|---|---|
A | B | C | D | E | |||
1 | −1 | 1 | 1 | −1 | 1 | 403.8 | 206.5 |
2 | −1 | −1 | −1 | −1 | −1 | 85.3 | 1143.8 |
3 | 1 | 1 | −1 | −1 | 1 | 134.21 | 1547.3 |
4 | 1 | 1 | −1 | 1 | −1 | 252.84 | 930.6 |
5 | −1 | −1 | −1 | 1 | −1 | 130.7 | 922.9 |
6 | 1 | 1 | 1 | −1 | −1 | 41.5 | 2030.5 |
7 | 1 | −1 | −1 | −1 | −1 | 135.07 | 2084.8 |
8 | 1 | 1 | −1 | −1 | 1 | 298.53 | 1875.9 |
9 | 1 | −1 | −1 | −1 | −1 | 88.78 | 1154.7 |
10 | 1 | −1 | −1 | −1 | 1 | 133.2 | 916.4 |
11 | 1 | −1 | −1 | 1 | −1 | 103.9 | 1143.9 |
12 | −1 | 1 | 1 | 1 | 1 | 110.74 | 2775.8 |
13 | −1 | 1 | −1 | −1 | −1 | 129.56 | 1152.4 |
14 | −1 | 1 | −1 | −1 | 1 | 228.5 | 925.3 |
15 | 1 | 1 | −1 | 1 | 1 | 399.6 | 4375.9 |
16 | 1 | −1 | −1 | −1 | 1 | 123.2 | 2753.9 |
17 | −1 | 1 | −1 | 1 | 1 | 219.5 | 1401.6 |
18 | 1 | 1 | 1 | −1 | −1 | 145.12 | 1170.5 |
19 | −1 | 1 | 1 | 1 | −1 | 441.45 | 920.5 |
20 | −1 | −1 | 1 | −1 | 1 | 309.3 | 1382.7 |
21 | 1 | −1 | 1 | 1 | 1 | 150.09 | 3640.4 |
22 | −1 | −1 | −1 | −1 | 1 | 125.8 | 1106.1 |
23 | 1 | 1 | 1 | 1 | −1 | 375.5 | 915.6 |
24 | 1 | 1 | 1 | 1 | 1 | 259.6 | 1210.3 |
25 | −1 | −1 | 1 | −1 | −1 | 346.3 | 1826.6 |
26 | −1 | −1 | 1 | 1 | 1 | 171.5 | 1154.6 |
27 | 1 | −1 | 1 | −1 | 1 | 108.5 | 1163.7 |
28 | −1 | −1 | 1 | 1 | −1 | 66.7 | 2982.6 |
29 | −1 | 1 | 1 | −1 | −1 | 90.73 | 1844.9 |
30 | −1 | −1 | −1 | 1 | 1 | 145.74 | 1867.4 |
31 | −1 | 1 | −1 | 1 | −1 | 497.4 | 1133.8 |
32 | 1 | −1 | 1 | 1 | −1 | 78.93 | 4778.2 |