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Table 1 Response of two-level full factorial design for screening of variables for CMCase and protease production

From: Novel Bacillus subtilis IND19 cell factory for the simultaneous production of carboxy methyl cellulase and protease using cow dung substrate in solid-substrate fermentation

Run Sucrose Peptone NaH2PO4 pH Moisture CMCase activity (U/g) Protease activity (U/g)
A B C D E
1 −1 1 1 −1 1 403.8 206.5
2 −1 −1 −1 −1 −1 85.3 1143.8
3 1 1 −1 −1 1 134.21 1547.3
4 1 1 −1 1 −1 252.84 930.6
5 −1 −1 −1 1 −1 130.7 922.9
6 1 1 1 −1 −1 41.5 2030.5
7 1 −1 −1 −1 −1 135.07 2084.8
8 1 1 −1 −1 1 298.53 1875.9
9 1 −1 −1 −1 −1 88.78 1154.7
10 1 −1 −1 −1 1 133.2 916.4
11 1 −1 −1 1 −1 103.9 1143.9
12 −1 1 1 1 1 110.74 2775.8
13 −1 1 −1 −1 −1 129.56 1152.4
14 −1 1 −1 −1 1 228.5 925.3
15 1 1 −1 1 1 399.6 4375.9
16 1 −1 −1 −1 1 123.2 2753.9
17 −1 1 −1 1 1 219.5 1401.6
18 1 1 1 −1 −1 145.12 1170.5
19 −1 1 1 1 −1 441.45 920.5
20 −1 −1 1 −1 1 309.3 1382.7
21 1 −1 1 1 1 150.09 3640.4
22 −1 −1 −1 −1 1 125.8 1106.1
23 1 1 1 1 −1 375.5 915.6
24 1 1 1 1 1 259.6 1210.3
25 −1 −1 1 −1 −1 346.3 1826.6
26 −1 −1 1 1 1 171.5 1154.6
27 1 −1 1 −1 1 108.5 1163.7
28 −1 −1 1 1 −1 66.7 2982.6
29 −1 1 1 −1 −1 90.73 1844.9
30 −1 −1 −1 1 1 145.74 1867.4
31 −1 1 −1 1 −1 497.4 1133.8
32 1 −1 1 1 −1 78.93 4778.2