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Table 2 Analysis of variance (ANOVA) for the CMCase and protease activity of B. subtilis IND19

From: Novel Bacillus subtilis IND19 cell factory for the simultaneous production of carboxy methyl cellulase and protease using cow dung substrate in solid-substrate fermentation

Source Sum of squares df Mean square F value p value  
Analysis of variance (ANOVA) for the CMCase activity of B. subtilis IND19
 Model 4.72E+05 27 1.75E+04 49.75 0.0008 Significant
 A-Sucrose 1.42E+04 1 1.42E+04 40.46 0.0031  
 B-Peptone 93049.74 1 93049.74 264.84 <0.0001  
 C-NaH2PO4 3.27E+03 1 3.27E+03 9.31 0.038  
 D-pH 1.73E+04 1 1.73E+04 49.13 0.0022  
 E-Moisture 3.24E+03 1 3.24E+03 8.66 0.0423  
 AB 1.87E+03 1 1.87E+03 5.33 0.0821  
 AC 5.85E+03 1 5.85E+03 16.64 0.0151  
 AD 1.18E+04 1 1.18E+04 33.57 0.0044  
 AE 6.57E+03 1 6.57E+03 18.7 0.0124  
 BC 8.98E+03 1 8.98E+03 25.56 0.0072  
 BD 6.36E+04 1 6.36E+04 180.91 0.0002  
 CD 1.90E+04 1 1.90E+04 54.15 0.0018  
 CE 4.42E+03 1 4.42E+03 12.58 0.0239  
 DE 3.30E+04 1 3.30E+04 93.8 0.0006  
 ABC 3.46E+03 1 3.46E+03 9.86 0.0348  
 ABE 7.46E+03 1 7.46E+03 21.23 0.01  
 ACD 1.94E+04 1 1.94E+04 55.17 0.0018  
 ACE 1.63E+04 1 1.63E+04 46.53 0.0024  
 ADE 1.91E+04 1 1.91E+04 54.34 0.0018  
 BCD 2.19E+03 1 2.19E+03 6.23 0.0671  
 BCE 4.10E+03 1 4.10E+03 11.68 0.0268  
 BDE 6.52E+04 1 6.52E+04 185.67 0.0002  
 ABCE 1.36E+04 1 1.36E+04 38.81 0.0034  
 ABDE 2.15E+04 1 2.15E+04 61.14 0.0014  
 ACDE 1.35E+03 1 1.35E+03 3.84 0.1216  
 BCDE 8.84E+03 1 8.84E+03 25.15 0.0074  
 ABCDE 3.54E+03 1 3.54E+03 10.08 0.0337  
 Residual 1.41E+03 4 1.41E+03    
 Cor Total 4.73E+05 31 4.73E+05    
ANOVA for the protease activity of B. subtilis IND19
 Model 3.36E+07 23 1.46E+06 75.06 <0.0001 Significant
 A-Sucrose 3.93E+05 1 3.93E+05 20.22 0.002  
 B-Peptone 2.84E+06 1 2.84E+06 145.89 <0.0001  
 C-NaH2PO4 3.63E+06 1 3.63E+06 186.53 <0.0001  
 D-pH 2.79E+06 1 2.79E+06 143.25 <0.0001  
 E-Moisture 2.02E+05 1 2.02E+05 10.37 0.0122  
 AB 1.65E+06 1 1.65E+06 84.73 <0.0001  
 AC 2.17E+05 1 2.17E+05 11.14 0.0103  
 AD 8.21E+05 1 8.21E+05 42.21 0.0002  
 AE 1.27E+05 1 1.27E+05 6.55 0.0337  
 BC 9.99E+04 1 9.99E+04 5.14 0.0532  
 BD 3.81E+06 1 3.81E+06 195.77 <0.0001  
 BE 2.28E+06 1 2.28E+06 117.25 <0.0001  
 CE 1.05E+05 1 1.05E+05 5.38 0.0489  
 ABD 1.11E+06 1 1.11E+06 57.04 <0.0001  
 ABE 4.54E+06 1 4.54E+06 233.39 <0.0001  
 ACE 2.27E+05 1 2.27E+05 11.68 0.0091  
 ADE 2.25E+06 1 2.25E+06 115.41 <0.0001  
 BCD 1.42E+06 1 1.42E+06 72.82 <0.0001  
 BCE 5.99E+05 1 5.99E+05 30.8 0.0005  
 ABCD 6.30E+05 1 6.30E+05 32.38 0.0005  
 ABCE 1.25E+06 1 1.25E+06 64.02 <0.0001  
 ACDE 2.42E+06 1 2.42E+06 124.24 <0.0001  
 ABCDE 1.98E+05 1 1.98E+05 10.17 0.0128  
 Residual 1.56E+05 8     
 Cor Total 3.37E+07 31