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Table 3 Central composite design of the medium component in coded units for CMCase and protease production

From: Novel Bacillus subtilis IND19 cell factory for the simultaneous production of carboxy methyl cellulase and protease using cow dung substrate in solid-substrate fermentation

Std

A:pH

B:Peptone

C:NaH2PO4

Enzyme activity (/g)

Cellulase

Protease

1

0

0

0

464

4040

2

−1

−1

−1

270

2080

3

0

0

0

440

4163

4

0

0

1.682

348

3942

5

1.682

0

0

370

1798

6

1

1

1

418

4080

7

1

−1

−1

295

4019

8

0

0

0

442

4530

9

−1

−1

1

180

1728

10

0

0

0

462

4120

11

−1

1

1

362

4686

12

1

1

−1

298

3501

13

1

−1

1

320

398

14

0

1.682

0

434

4000

15

0

0

−1.682

152

4611

16

0

0

0

429

4200

17

−1.682

0

0

127

1608

18

−1

−1

−1

79

3263

19

0

0

0

462

4180

20

0

−1.682

0

252

2109