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Table 3 Central composite design of the medium component in coded units for CMCase and protease production

From: Novel Bacillus subtilis IND19 cell factory for the simultaneous production of carboxy methyl cellulase and protease using cow dung substrate in solid-substrate fermentation

Std A:pH B:Peptone C:NaH2PO4 Enzyme activity (/g)
Cellulase Protease
1 0 0 0 464 4040
2 −1 −1 −1 270 2080
3 0 0 0 440 4163
4 0 0 1.682 348 3942
5 1.682 0 0 370 1798
6 1 1 1 418 4080
7 1 −1 −1 295 4019
8 0 0 0 442 4530
9 −1 −1 1 180 1728
10 0 0 0 462 4120
11 −1 1 1 362 4686
12 1 1 −1 298 3501
13 1 −1 1 320 398
14 0 1.682 0 434 4000
15 0 0 −1.682 152 4611
16 0 0 0 429 4200
17 −1.682 0 0 127 1608
18 −1 −1 −1 79 3263
19 0 0 0 462 4180
20 0 −1.682 0 252 2109