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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases

Fig. 3

Verifying the presence or absence of a transglycosylation product. The reaction products were analyzed by TLC on activated silica plates by using a n-butanol-n-propanol-ethanol–water eluent (2:3:3:2). a Enzymatic product analysis of substrates cellobiose (Clbs) and pNPGlc at 10 and 20 mM and measured after 5 min and 4 h. Lane 1 and 2 represent the products of the 10 mM Clbs reaction after 5 min and 4 h, respectively. Lanes 3 and 4 represent the products of the 20 mM Clbs reaction after 5 min and the 4 h, respectively. Lanes 5 and 6 represent the products of the 10 mM pNPGlc reaction after 5 min and 4 h, respectively. Lanes 7 and 8 represent the products of the 20 mM pNPGlc reaction after 5 min and 4 h, respectively. No transoligosaccharide products band was observed in any one of the reactions indicating absence of the transglycosylation. b Similar assays were done as in a, but in the presence of Glc. Lanes 1–4 indicates glucose concentration ranging from 0.25, 0.5, 0.75, 1 M Glc, respectively, for 5 min. Lanes 5–8 indicates glucose concentration ranging from 0.25, 0.5, 0.75, and 1 M Glc, respectively, for 4 h. No transoligosaccharide products band was observed in any of the reaction indicating absence of transglycosylation reaction in the presence of exogenous glucose

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