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Table 1 Comparison of the kinetic parameters of H0HC94 with different BG substrates

From: Using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases

Organisms

Topt,°C

pHopt

Substrates

K m, mM

k cat, s−1

k cat/K m (mM−1s−1)

H0HC94 (Agrobacterium tumefaciens 5A)

52

7.2

pNPGlc

3.09 ± 0.40

277.9 ± 4

89.94

pNPGal

12.93 ± 2.00

214.9 ± 7

16.62

Clbs

2.94 ± 0.20

233.4 ± 6

79.32

  1. All assays were performed as indicated in “Experimental” section