Glucose (mM) |
K
m (mM) |
k
cat (s−1) |
k
cat/K
m
|
K
i, app (mM) | ΔT
m (°C) |
t
1/2 (min) |
---|
0 | 3.09 | 277.93 | 89.94 | – | 0 | 16 |
100 | 3.55 | 236.71 | 66.67 | 686 | 2.4 ± 0.19 | 38 |
200 | 2.64 | 207.79 | 78.70 | 3.3 ± 0.06 | 44 |
400 | 1.89 | 171.98 | 90.99 | 6.7 ± 0.21 | 160 |
600 | 1.68 | 138.33 | 82.33 | 7.7 ± 0.16 | 289 |
800 | 1.37 | 120.31 | 113.37 | 8.7 ± 0.26 | 310 |
- All assays were performed as indicated in “Experimental” section
- The standard deviation of k
cat and K
m are within 5 % of the original values
- The Tm of enzyme in the absence of glucose was 53.73 ± 0.2. For ΔT
m, the T
m in the absence of glucose was subtracted from each data
- All half-life measurements were measured in triplicate and repeated at least twice