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Table 2 The effect of glucose on the steady-state kinetic parameters (K m, k cat, K i, app), melting temperature (Tm), and half-life (t 1/2) on HOHC94

From: Using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases

Glucose (mM) K m (mM) k cat (s−1) k cat/K m K i, app (mM) ΔT m (°C) t 1/2 (min)
0 3.09 277.93 89.94 0 16
100 3.55 236.71 66.67 686 2.4 ± 0.19 38
200 2.64 207.79 78.70 3.3 ± 0.06 44
400 1.89 171.98 90.99 6.7 ± 0.21 160
600 1.68 138.33 82.33 7.7 ± 0.16 289
800 1.37 120.31 113.37 8.7 ± 0.26 310
  1. All assays were performed as indicated in “Experimental” section
  2. The standard deviation of k cat and K m are within 5 % of the original values
  3. The Tm of enzyme in the absence of glucose was 53.73 ± 0.2. For ΔT m, the T m in the absence of glucose was subtracted from each data
  4. All half-life measurements were measured in triplicate and repeated at least twice