|
Glucose (mM)
|
K
m (mM)
|
k
cat (s−1)
|
k
cat/K
m
|
K
i, app (mM)
|
ΔT
m (°C)
|
t
1/2 (min)
|
|---|
|
0
|
3.09
|
277.93
|
89.94
|
–
|
0
|
16
|
|
100
|
3.55
|
236.71
|
66.67
|
686
|
2.4 ± 0.19
|
38
|
|
200
|
2.64
|
207.79
|
78.70
|
3.3 ± 0.06
|
44
|
|
400
|
1.89
|
171.98
|
90.99
|
6.7 ± 0.21
|
160
|
|
600
|
1.68
|
138.33
|
82.33
|
7.7 ± 0.16
|
289
|
|
800
|
1.37
|
120.31
|
113.37
|
8.7 ± 0.26
|
310
|
- All assays were performed as indicated in “Experimental” section
- The standard deviation of k
cat and K
m are within 5 % of the original values
- The Tm of enzyme in the absence of glucose was 53.73 ± 0.2. For ΔT
m, the T
m in the absence of glucose was subtracted from each data
- All half-life measurements were measured in triplicate and repeated at least twice
