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Table 2 The effect of glucose on the steady-state kinetic parameters (K m, k cat, K i, app), melting temperature (Tm), and half-life (t 1/2) on HOHC94

From: Using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases

Glucose (mM)

K m (mM)

k cat (s−1)

k cat/K m

K i, app (mM)

ΔT m (°C)

t 1/2 (min)

0

3.09

277.93

89.94

0

16

100

3.55

236.71

66.67

686

2.4 ± 0.19

38

200

2.64

207.79

78.70

3.3 ± 0.06

44

400

1.89

171.98

90.99

6.7 ± 0.21

160

600

1.68

138.33

82.33

7.7 ± 0.16

289

800

1.37

120.31

113.37

8.7 ± 0.26

310

  1. All assays were performed as indicated in “Experimental” section
  2. The standard deviation of k cat and K m are within 5 % of the original values
  3. The Tm of enzyme in the absence of glucose was 53.73 ± 0.2. For ΔT m, the T m in the absence of glucose was subtracted from each data
  4. All half-life measurements were measured in triplicate and repeated at least twice