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Table 3 The effect of IL (0.9 M) on the steady-state kinetic parameters (V max, K m, k cat) of H0HC94 (E) in the absence and the presence of 100 mM Glc

From: Using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases

Reaction

V max (mM)

k cat (s−1)

K m (mM)

k cat/K m (s−1 mM−1)

E

0.31

277.93

3.09

89.94

E + [C2C1im][C1C1PO4]

0.43

188.11

2.17

86.49

E + [C2C1im][C1C1PO4] + 0.1 M Glc

0.27

116.71

1.50

77.49

E + [C2C1im][C2C2PO4]

0.38

165.79

4.20

39.43

E + [C2C1im][C2C2PO4] + 0.1 M Glc

0.25

107.50

2.59

41.60

E + [C2C1im][MeCHOHCO2]

0.33

143.56

13.26

10.83

E + [C2C1im][MeCHOHCO2] + 0.1 M Glc

0.26

113.16

12.31

9.19

  1. The standard deviation of k cat and K m are within 5 % of the original values
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Biotechnology for Biofuels and Bioproducts

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