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Table 3 The effect of IL (0.9 M) on the steady-state kinetic parameters (V max, K m, k cat) of H0HC94 (E) in the absence and the presence of 100 mM Glc

From: Using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases

Reaction V max (mM) k cat (s−1) K m (mM) k cat/K m (s−1 mM−1)
E 0.31 277.93 3.09 89.94
E + [C2C1im][C1C1PO4] 0.43 188.11 2.17 86.49
E + [C2C1im][C1C1PO4] + 0.1 M Glc 0.27 116.71 1.50 77.49
E + [C2C1im][C2C2PO4] 0.38 165.79 4.20 39.43
E + [C2C1im][C2C2PO4] + 0.1 M Glc 0.25 107.50 2.59 41.60
E + [C2C1im][MeCHOHCO2] 0.33 143.56 13.26 10.83
E + [C2C1im][MeCHOHCO2] + 0.1 M Glc 0.26 113.16 12.31 9.19
  1. The standard deviation of k cat and K m are within 5 % of the original values