Using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases

Table 4 Melting temperature (T _m) of H0HC94 in 0.9 M IL’s, DMF, and NaCl, both in the absence and presence of 100 mM glucose (Glc)

	T_m(°C) in 0.9 M IL	∆T_m	T_m(°C) in 0.9 M IL + 100 mM Glc	∆T_m
H0HC94 (E)	53.73 ± 0.40	0.0	–	–
E + Glc	54.93 ± 0.85	1.20	–	–
E + [C₂C₁im][MeSO₃]	46.54 ± 0.68	−7.19	48.37 ± 1.38	−5.36
E + [C₂C₁im][C₂C₂PO₄]	44.64 ± 1.90	−9.09	46.73 ± 1.85	−7.00
E + [C₂C₁im][MeCO₂]	44.50_- ± 1.62	−9.23	46.58 ± 1.18	−7.15
E + [C₂C₁im][C₁C₁PO₄]	48.44 ± 2.50	−5.29	49.24 ± 1.14	−4.49
E + [C₂C₁im][MeCHOHCO₂]	44.86 ± 1.50	−8.87	47.99 ± 1.85	−5.74
E + [C₂C₁im][Cl]	44.87 ± 1.13	−8.86	45.30 ± 0.59	−8.43
E + DMF	44.21 ± 0.44	−9.52	45.40 ± 2.21	−8.33

∆T_m was calculated by subtracting the zero IL H0HC94 T_m (53.73 °C) from the T_m of H0HC94 in 0.9 M IL and also from the T_m of H0HC94 0.9 M IL and Glc co-solvent. Data are expressed in °C and represent mean ± SD of three independent experiments, each performed in triplicate