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Table 4 Melting temperature (T m) of H0HC94 in 0.9 M IL’s, DMF, and NaCl, both in the absence and presence of 100 mM glucose (Glc)

From: Using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases

  Tm(°C) in 0.9 M IL ∆Tm Tm(°C) in 0.9 M IL + 100 mM Glc ∆Tm
H0HC94 (E) 53.73 ± 0.40 0.0 – –
E + Glc 54.93 ± 0.85 1.20 – –
E + [C2C1im][MeSO3] 46.54 ± 0.68 −7.19 48.37 ± 1.38 −5.36
E + [C2C1im][C2C2PO4] 44.64 ± 1.90 −9.09 46.73 ± 1.85 −7.00
E + [C2C1im][MeCO2] 44.50- ± 1.62 −9.23 46.58 ± 1.18 −7.15
E + [C2C1im][C1C1PO4] 48.44 ± 2.50 −5.29 49.24 ± 1.14 −4.49
E + [C2C1im][MeCHOHCO2] 44.86 ± 1.50 −8.87 47.99 ± 1.85 −5.74
E + [C2C1im][Cl] 44.87 ± 1.13 −8.86 45.30 ± 0.59 −8.43
E + DMF 44.21 ± 0.44 −9.52 45.40 ± 2.21 −8.33
  1. ∆Tm was calculated by subtracting the zero IL H0HC94 Tm (53.73 °C) from the Tm of H0HC94 in 0.9 M IL and also from the Tm of H0HC94 0.9 M IL and Glc co-solvent. Data are expressed in °C and represent mean ± SD of three independent experiments, each performed in triplicate
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Biotechnology for Biofuels and Bioproducts

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