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Table 3 Effect of temperature on vanillin inhibition

From: Inhibition of lignin-derived phenolic compounds to cellulase

Temperature (ºC) Cellulose conversiona without vanillin (%) Cellulose conversion with vanillinb (%) Apparent inhibition (%) Inhibitor-binding constant β (vanillin, mL/mg)
30 22.0 ± 1.7 14.4 ± 0.2 34.4 ± 3.0 0.1048
40 35.7 ± 0.6 22.9 ± 0.5 35.9 ± 1.6 0.1121
50 41.4 ± 0.6 26.9 ± 0.6 35.0 ± 0.9 0.1092
  1. aEnzymatic hydrolysis of Avicel was carried out with cellulose concentration of 10 mg/mL and cellulase concentration of 0.3 mg/mL at pH 4.8. Cellulose conversion was determined at 24 h
  2. bVanillin concentration was 5 mg/mL