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Table 3 Effect of temperature on vanillin inhibition

From: Inhibition of lignin-derived phenolic compounds to cellulase

Temperature (ºC)

Cellulose conversiona without vanillin (%)

Cellulose conversion with vanillinb (%)

Apparent inhibition (%)

Inhibitor-binding constant β (vanillin, mL/mg)

30

22.0 ± 1.7

14.4 ± 0.2

34.4 ± 3.0

0.1048

40

35.7 ± 0.6

22.9 ± 0.5

35.9 ± 1.6

0.1121

50

41.4 ± 0.6

26.9 ± 0.6

35.0 ± 0.9

0.1092

  1. aEnzymatic hydrolysis of Avicel was carried out with cellulose concentration of 10 mg/mL and cellulase concentration of 0.3 mg/mL at pH 4.8. Cellulose conversion was determined at 24 h
  2. bVanillin concentration was 5 mg/mL
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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