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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Specific tracking of xylan using fluorescent-tagged carbohydrate-binding module 15 as molecular probe

Fig. 1

Plasmid map (a), construction scheme and pictorial representation (b) of the OC15 probe. mOrange2 C-terminal end is linked to the N-terminal end of CBM15 (Cellvibrio japonicus). The red rectangle represents N-terminal six histidines tag. The red sphere represent the metal ion in CBM15. The structural model shown was constructed using PDB files 1GNY and 2H5O (obtained for the closely related mOrange fluorescent protein). The sequence linking the fluorescent protein to the CBM is composed of a glycine residue

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