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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Crystal structure and biochemical characterization of the recombinant ThBgl, a GH1 β-glucosidase overexpressed in Trichoderma harzianum under biomass degradation conditions

Fig. 1

Gene mapping and quantification of the expression of β-glucosidases from T. harzianum using RNA-Seq data. The genes encoding the GH1 and GH3 β-glucosidases that were used for read mapping were identified from the assembled genome of the T. harzianum T6776 strain (GenBank access number JOKZ00000000.1) (Additional file 1: Table S1) and compared to the RNA-Seq libraries from T. harzianum IOC-3844 cultured with LAC, CEL, or DSB. The squalene epoxidase (GenBank: AM050097.1), TATA box-binding protein (GenBank: KKP02269.1) and the 13-kDa ribonucleoprotein (RNP)-associated protein (GenBank: KKO98059.1) sequences were used for the normalization of gene expression. RPKM reads per kilobase per million mapped reads

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