Fig. 3

Biochemical properties of purified rThBgl. a Optimal temperature screening for rThBgl activity. b pH dependence of rThBgl activity in a 100 mM citrate/phosphate/glycine buffer with a pH range from 2 to 10. c The kinetic curves of pNPG (0–10 mM). d The kinetic curves of cellobiose (0–10 mM). For kinetic analysis, the reaction mixture contained 100 mM sodium phosphate buffer pH 6.0, and all reactions were incubated for 10 min at 40 °C