Fig. 1From: Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclizationHigh-expression yield and purity of target fusion proteins were achieved. Products were subjected to SDS-PAGE analysis, before a and after b the cyclization. Lanes: lane M marker; lane 1 the soluble lysates; lane 2 supernatants after the first-round ITC; lane 3 supernatants undergoing the second-round ITCBack to article page