Fig. 2

a SDS-PAGE analysis of proteolytic digestion products of cyclized lichenase. Lanes lane M marker; lane 1 purified cyclized lichenase before digestion; lane 2 the corresponding products after digestion. The band with an apparent molecular weight of 48 kDa is the TEV protease. b and c MALDI-TOF mass spectrum of lichenase undergoing cyclization before b and after c TEV cleavage