Fig. 6

The profiles of plasmid pET-Cylized lichenase (a) and pET-linear lichenase (b). a The TEV cleavage site was inserted between BglS gene and SpyCatcher gene. The cyclized lichenase gene was cloned between NedI and HindIII digestion sites in pET 22(b+), while b the linear lichenase fusion gene was cloned into pET 22(b+) using NedI and HindIII as restriction enzymes as well