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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclization

Fig. 6

The profiles of plasmid pET-Cylized lichenase (a) and pET-linear lichenase (b). a The TEV cleavage site was inserted between BglS gene and SpyCatcher gene. The cyclized lichenase gene was cloned between NedI and HindIII digestion sites in pET 22(b+), while b the linear lichenase fusion gene was cloned into pET 22(b+) using NedI and HindIII as restriction enzymes as well

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