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Table 1 Purification profile of the linear and cyclized lichenase

From: Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclization

 

Linear lichenase

Cyclized lichenase

Before ITC

After ITC

Before ITC

After ITC

Activity (U)

8620.88

1911.94

3768.75

638.70

Protein (mg)

303.98

15.08

339.3

11.05

Specific activity (U/mg)

28.36

126.78

11.10

57.73

specific productivity (U/g)a

3533.15

783.58

1544.57

261.76

Activity recovry (%)

22.18

16.95

Purification fold

4.47

5.20

  1. The table data were based on 1L culture
  2. a The specific productivity means the activity productivity of dry cell weight (1 L culture)
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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