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Table 2 Kinetic parameters of the linear and cyclized lichenase

From: Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclization

  Vmax (μmol/min/mL) Km (mg/mL) Kcat (s−1) Kcat/Km (L/mg/s)
Linear lichenase 4.30 ± 0.47 12.96 ± 1.93 141.14 ± 16.28 10.36 ± 2.43
Cyclized lichenase 2.65 ± 0.16 7.58 ± 0.92 213.2 ± 23.43 28.1 ± 3.44
  1. Experiments were performed in triplicate and error means the standard deviation
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