Skip to main content
Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Engineering of a high lipid producing Yarrowia lipolytica strain

Fig. 3

Overexpression of DGA2 genes in Y. lipolytica. Six DGA2 genes (Table 2) under the control of the Y. lipolytica TEF1 promoter were overexpressed in NS125 (a) and NS281 (b) strains that overexpress DGA1 from Y. lipolytica and R. toruloides, respectively. Fifteen transformants for each gene were analyzed by fluorescence-based lipid assay after 72 h of growth in nitrogen-limited media. The average with standard deviation from triplicate experiments is shown for the parent strain. Fluorescence was measured at excitation 486 nm and emission 510 nm and normalized by cell optical density (OD) at 600 nm

Back to article page
\