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Table 1 Relative lipid contents of strains with overexpressed or deleted genetic targets in addition to DGA1 overexpression

From: Engineering of a high lipid producing Yarrowia lipolytica strain

Genetic background Function (Fl/OD)mutant/(Fl/OD)NS125
WT Wild-type Yarrowia lipolytica strain 0.55 ± 0.02
DGA1 (NS125) Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor 1.00 ± 0.09
DGA1 Δgut2 Mitochondrial glycerol-3-phosphate dehydrogenase 0.95 ± 0.12
DGA1 GPD1 NAD-dependent glycerol-3-phosphate dehydrogenase 1.12 ± 0.03
DGA1 ME Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate 0.92 ± 0.15
DGA1 ACL1/2 ATP citrate lyase 1 & 2 0.99 ± 0.09
DGA1 ACC1 Acetyl-CoA carboxylase subunit 1 1.05 ± 0.19
DGA1 SCT1 Bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase 1.03 ± 0.01
DGA1 SLC1 1-acylglycerol-3-phosphate O-acyltransferase 1.25 ± 0.07
DGA1 SCD1 Stearoyl-CoA desaturase. Endoplasmic reticulum (ER) protein that catalyzes the Δ9-cis desaturation of saturated fatty acids 0.85 ± 0.18
DGA1 DGA2 DGAT1 acyl-CoA:diacylglycerol acyltransferase family 1.62 ± 0.03
DGA1 LRO1 Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis 1.33 ± 0.07
DGA1 Δtgl3 Bifunctional triacylglycerol lipase. Major lipid particle-localized triacylglycerol (TAG) lipase 0.79 ± 0.24
DGA1 Δtgl4 Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2 + -independent phospholipase A2 1.03 ± 0.08
DGA1 Δmfe1 Multifunctional enzyme, member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family 0.89 ± 0.03
  1. Lipid content was measured by fluorescence-based lipid assay after 96 h of fermentation in 48-well plates and normalized by the value corresponding to the parental strain NS125 overexpressing DGA1 alone. All genes described here were amplified from Y. lipolytica genomic DNA and sequences are given in Additional file 1
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