Fig. 4
From: Microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase
Improving propane production by rational design aiming at the active center of ADO. a Residues selected for mutagenesis. b Whole-cell assay of enzymes activity. Wild-type ADO is set at 100 %. A detailed protocol is provided in materials and methods. c In vitro enzymatic assay of mutants I127G, I127G/A48G. Wild-type ADO is set at 100 %. d De novo biosynthesis of propane using efficiency-enhanced mutants