Table 1 Quantitative RAPs and digested cellulose percentages of pretreated cellulose hydrolyzed by EG (+CBH I/β-G), CBH I, CBH I/β-G, and whole enzyme

EG + pretreated cellulose (65.48 %)

 Time (min)

0

71

204

340

–

 Recognition area (%)

42.47

42.02

40.96

41.59

–

 RAP (%)

64.85

64.17

62.55

63.51

–

 Digested cellulose (%)

0

1

3.5

2

–

+CBH I/β-G

 Time (min)

–

508

673

825

1085

1163

 Recognition area (%)

–

34.8

30.49

21.6

5.02

4.97

 RAP (%)

–

53.26

46.56

32.98

7.66

7.59

 Digested cellulose (%)

–

17.8

28.2

49.1

88.1

88.2

CBH I + pretreated cellulose (67 %)

 Time (min)

0

60

180

210

340

–

 Recognition area (%)

53.35

49.3

48.83

48.8

48.4

–

 RAP (%)

79.62

73.5

72.88

72.8

72.23

–

 Digested cellulose (%)

0

7.7

8.5

8.6

9.2

–

CBH I/β-G + pretreated cellulose (61.32 %)

 Time (min)

0

29

66

132

157

182

231

293

345

–

 Recognition area (%)

48.56

32.42

26.84

15.51

12.6

12

8.9

5.25

5.19

–

 RAP (%)

79.19

52.87

43.77

25.29

20.54

19.56

14.51

8.56

8.46

–

 Digested cellulose (%)

0

33.2

44.7

68

74

75.3

81.7

89.2

89.3

–

Whole enzyme + pretreated cellulose (65.65 %)

 Time (min)

0

12

41

109

156

189

237

344

–

 Recognition area (%)

43.16

38.04

28.17

19.4

13.24

6.01

5.13

4.34

–

 RAP (%)

65.74

57.94

42.9

29.55

20.16

9.15

7.81

6.61

–

 Digested cellulose (%)

0

11.8

34.7

36.19

69.3

86

88.1

89.9

–