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Table 1 Quantitative RAPs and digested cellulose percentages of pretreated cellulose hydrolyzed by EG (+CBH I/β-G), CBH I, CBH I/β-G, and whole enzyme

From: Real-time single molecular study of a pretreated cellulose hydrolysis mode and individual enzyme movement

EG + pretreated cellulose (65.48 %)
 Time (min) 0 71 204 340          
 Recognition area (%) 42.47 42.02 40.96 41.59          
 RAP (%) 64.85 64.17 62.55 63.51          
 Digested cellulose (%) 0 1 3.5 2          
+CBH I/β-G
 Time (min)          508 673 825 1085 1163
 Recognition area (%)          34.8 30.49 21.6 5.02 4.97
 RAP (%)          53.26 46.56 32.98 7.66 7.59
 Digested cellulose (%)          17.8 28.2 49.1 88.1 88.2
CBH I + pretreated cellulose (67 %)
 Time (min) 0 60 180 210 340         
 Recognition area (%) 53.35 49.3 48.83 48.8 48.4         
 RAP (%) 79.62 73.5 72.88 72.8 72.23         
 Digested cellulose (%) 0 7.7 8.5 8.6 9.2         
CBH I/β-G + pretreated cellulose (61.32 %)
 Time (min) 0 29 66 132 157 182 231 293 345     
 Recognition area (%) 48.56 32.42 26.84 15.51 12.6 12 8.9 5.25 5.19     
 RAP (%) 79.19 52.87 43.77 25.29 20.54 19.56 14.51 8.56 8.46     
 Digested cellulose (%) 0 33.2 44.7 68 74 75.3 81.7 89.2 89.3     
Whole enzyme + pretreated cellulose (65.65 %)
 Time (min) 0 12 41 109 156 189 237 344      
 Recognition area (%) 43.16 38.04 28.17 19.4 13.24 6.01 5.13 4.34      
 RAP (%) 65.74 57.94 42.9 29.55 20.16 9.15 7.81 6.61      
 Digested cellulose (%) 0 11.8 34.7 36.19 69.3 86 88.1 89.9      
  1. The total surface areas of imaged pretreated cellulose before the hydrolysis experiments were 65.48, 67, 61.32 and 65.65 %, respectively