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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Model-based optimization and scale-up of multi-feed simultaneous saccharification and co-fermentation of steam pre-treated lignocellulose enables high gravity ethanol production

Fig. 2

Optimization of seed cultivation in wheat straw pre-treatment liquor medium. a Effect of pre-treatment liquor concentration (% v/v) on cell yield after 24 h shake flask batch cultivation. The asterisks indicate that yeast growth was not sufficient to calculate the yield. Error bars indicate standard deviation of duplicate experiments. b Effects of the feed rate and the pre-treatment liquor concentration (% v/v) in feed medium on cell growth during fed-batch cultivation. Fed batch was started after a 24-h batch phase in 20 % (v/v) pre-treatment liquor medium. B_Yield, cell yields over the batch phase. FB_Yield, overall cell yield over the fed-batch phase, FB_Productivity, average cell productivity over the fed-batch phase. c Fermentation capacity of cells after batch and fed-batch cultivation. The conditions for seed cultivation in the subsequent multi-feed experiments were 20 % (v/v) pre-treatment liquor batch cultivation, followed by 50 % (v/v) pre-treatment liquor fed-batch cultivation at feed rate of 0.05 h−1

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