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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor endophyticus suitable for biodiesel synthesis

Fig. 3

Time-course profile of secretory expression of ReLipA in P. pastoris by high cell-density fermentation (a), and SDS-PAGE analysis of the secreted proteins during the fermentation process (b). (Filled square) lipase activity; (filled circle) cell wet weight; and (filled triangle) protein concentration. Lane M, low molecular weight standard proteins; lane 1 before methanol induction; lanes 2–6 culture supernatants after 24, 48, 72, 96, and 120 h of induction, respectively

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