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Table 1 Purification summary of the recombinant lipase (ReLipA) from R. endophyticus expressed in P. pastoris

From: Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor endophyticus suitable for biodiesel synthesis

Purification step Total activity (U)a Total protein (mg)b Specific activity (U mg−1) Purification (fold) Yield (%)
Crude enzyme 11711 10.6 1099 1.0 100
SP-Sepharose 8871 7.6 1169 1.1 75.7
  1. aEnzyme’s activity was determined in 50 mM citrate buffer (pH 6.0) at 40 °C for 10 min using pNPL as the substrate
  2. bProtein concentration was measured by the method of Lowry et al. [43] using BSA as the standard