Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor endophyticus suitable for biodiesel synthesis

Table 1 Purification summary of the recombinant lipase (ReLipA) from R. endophyticus expressed in P. pastoris

Purification step	Total activity (U)^a	Total protein (mg)^b	Specific activity (U mg⁻¹)	Purification (fold)	Yield (%)
Crude enzyme	11711	10.6	1099	1.0	100
SP-Sepharose	8871	7.6	1169	1.1	75.7

^aEnzyme’s activity was determined in 50 mM citrate buffer (pH 6.0) at 40 °C for 10 min using pNPL as the substrate
^bProtein concentration was measured by the method of Lowry et al. [43] using BSA as the standard

ISSN: 2731-3654