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Table 3 Kinetic parameters of ReLipA

From: Expression and characterization of a novel 1,3-regioselective cold-adapted lipase from Rhizomucor endophyticus suitable for biodiesel synthesis

Substrate K m (mM) V max (μmol min−1 mg−1) K cat (s−1) k cat/K m (mg−1 s−1)
pNPC (C8) 2.30 ± 0.21 1288.0 ± 48.5 0.891 0.387
pNPL (C12) 0.64 ± 0.06 385.3 ± 17.5 0.267 0.416
pNPM (C14) 0.10 ± 0.01 258.1 ± 10.5 0.179 1.79
  1. The kinetic parameters of ReLipA were determined at 40 °C in 50 mM citrate buffer (pH 6.0) for 5 min using different substrate concentrations