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Table 5 Kinetic parameters of CoGH1A and some reported microbial β-galactosidases with relatively high activity

From: A multifunctional thermophilic glycoside hydrolase from Caldicellulosiruptor owensensis with potential applications in production of biofuels and biochemicals

Resource

GH family

Substrate

K m (mM)

V max (μmol mg−1 min−1)

k cat (s−1)

k cat/K m (mM−1 s−1)

OT (°C)

OP

Reference

C. owensensis

GH1

pNPGal

0.607

5100

4522

7450

75–85

5.5

This work

Alicyclobacillus acidocaldarius

GH1

oNPGal

5.50

8045.5

1462.8

65

5.5

[35]

Hot spring metagenome

GH1

pNPGal

3.33

40.00

2000.0

600.6

65

8.0

[50]

Lactobacillus delbrueckii subsp. bulgaricus

GH2

oNPGal

0.919

317

603

655

45–60

7.5

[23]

Lactose

19.2

123

234

12.3

Bifidobacterium breve

GH2

oNPGal

1.3

486

937

722

50

7.0

[51]

Marine metagenomic library

GH2

Lactose

17.98

131.16

7.29

50

7.0

[52]

Bacillus licheniformis

GH42

oNPGal

13.7

299

785

57.3

50

6.5

[53]

C. saccharolyticus

GH42

oNPGal

1.21

149

123

80

6.0

[21]

Lactose

30.0

42

1.4

Thermotoga naphthophila

GH42

oNPGal

1.31

3385.67

90

6.8

[13]

Lactose

1.43

2.67

70

5.8

Thermotoga maritima

GH42

pNPGal

2.74

351

5.85

2.13

80

5.5

[54]

  1. OT optimum temperature; OP optimum pH; no data