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Table 5 Kinetic parameters of CoGH1A and some reported microbial β-galactosidases with relatively high activity

From: A multifunctional thermophilic glycoside hydrolase from Caldicellulosiruptor owensensis with potential applications in production of biofuels and biochemicals

Resource GH family Substrate K m (mM) V max (μmol mg−1 min−1) k cat (s−1) k cat/K m (mM−1 s−1) OT (°C) OP Reference
C. owensensis GH1 pNPGal 0.607 5100 4522 7450 75–85 5.5 This work
Alicyclobacillus acidocaldarius GH1 oNPGal 5.50 8045.5 1462.8 65 5.5 [35]
Hot spring metagenome GH1 pNPGal 3.33 40.00 2000.0 600.6 65 8.0 [50]
Lactobacillus delbrueckii subsp. bulgaricus GH2 oNPGal 0.919 317 603 655 45–60 7.5 [23]
Lactose 19.2 123 234 12.3
Bifidobacterium breve GH2 oNPGal 1.3 486 937 722 50 7.0 [51]
Marine metagenomic library GH2 Lactose 17.98 131.16 7.29 50 7.0 [52]
Bacillus licheniformis GH42 oNPGal 13.7 299 785 57.3 50 6.5 [53]
C. saccharolyticus GH42 oNPGal 1.21 149 123 80 6.0 [21]
Lactose 30.0 42 1.4
Thermotoga naphthophila GH42 oNPGal 1.31 3385.67 90 6.8 [13]
Lactose 1.43 2.67 70 5.8
Thermotoga maritima GH42 pNPGal 2.74 351 5.85 2.13 80 5.5 [54]
  1. OT optimum temperature; OP optimum pH; no data