A multifunctional thermophilic glycoside hydrolase from Caldicellulosiruptor owensensis with potential applications in production of biofuels and biochemicals

Table 6 Characteristic of GalOS synthesis by different β-galactosidases in batch process

Enzyme source	Initial lactose concentration (g L⁻¹)	Enzyme loading (U_lactose ml⁻¹ μg ml⁻¹)		Temperature (°C)	Time for reaching maximum yield	GalOS productivity (g L⁻¹ h⁻¹)	Maximum yield of GalOS %	References
Lactobacillus delbrueckii subsp. bulgaricus	205	1.5	>12.2^a	50	5 h	19.8	~50	[23]
Bifidobacterium breve	200	2.5	>25.8^a	30	6 h	14.7	44	[51]
Thermotoga maritima	500	1.5	>23.6^a	80	5 h	18.2	18	[39, 55]
Marine metagenomic library	360	18	267.9	40	~10 h	20.6	57.1	[52]
Caldicellulosiruptor owensensis	500	2.5	8.9	70	50 min	265.2	44.2	This work

^aThe given data were calculated using the values of the V _max of each enzyme as the specific activity

ISSN: 2731-3654