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Table 1 List of strains and plasmids used in this study

From: Co-production of hydrogen and ethanol by pfkA-deficient Escherichia coli with activated pentose-phosphate pathway: reduction of pyruvate accumulation

Strains and plasmids Description Source
SH5 E. coli BW25113 ΔhycA ΔhyaAB ΔhybBC ΔldhA ΔfrdAB [31]
SH5_ZG SH5/pEcZG This study
SH9 SH5ΔpfkA
SH9* SH9—adapted for anaerobic growth
SH9*_G SH9*/pEcG
SH9*_Z SH9*/pEcZ
SH9*_ZG SH9*/pEcZG
SH9*_ZGU SH9*/pEcZGU
SH10_ZG SH9* Δeddeda/pEcZG
Plasmids
 pDK7 Bacterial expression plasmid, Cm resistant [32]
 pEcG pDK7 carrying gnd of E. coli BW25113 [18]
 pEcZ pDK7 carrying zwf of E. coli BW25113
 pEcZG pDK7 carrying zwf, and gnd of E. coli BW25113
 pEcZGU pDK7 carrying zwf, gnd and udhA of E. coli BW25113 This study