Table 1 List of strains and plasmids used in this study
Strains and plasmids | Description | Source |
|---|---|---|
SH5 | E. coli BW25113 ΔhycA ΔhyaAB ΔhybBC ΔldhA ΔfrdAB | [31] |
SH5_ZG | SH5/pEcZG | This study |
SH9 | SH5ΔpfkA | |
SH9* | SH9—adapted for anaerobic growth | |
SH9*_G | SH9*/pEcG | |
SH9*_Z | SH9*/pEcZ | |
SH9*_ZG | SH9*/pEcZG | |
SH9*_ZGU | SH9*/pEcZGU | |
SH10_ZG | SH9* Δeddeda/pEcZG | |
Plasmids | ||
pDK7 | Bacterial expression plasmid, Cm resistant | [32] |
pEcG | pDK7 carrying gnd of E. coli BW25113 | [18] |
pEcZ | pDK7 carrying zwf of E. coli BW25113 | |
pEcZG | pDK7 carrying zwf, and gnd of E. coli BW25113 | |
pEcZGU | pDK7 carrying zwf, gnd and udhA of E. coli BW25113 | This study |