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Table 2 Comparison of metabolites yield of recombinant SH5, SH8*, and SH9* strains

From: Co-production of hydrogen and ethanol by pfkA-deficient Escherichia coli with activated pentose-phosphate pathway: reduction of pyruvate accumulation

Strainb

Overexpressed gene

Yield of metabolitesa,d (mol mol−1)

H2

Ethanol

Acetate

Pyruvate

SH5

–

1.44

0.79

0.67

–

zwf and gnd

1.60

1.09

0.35

–

SH8*c

–

1.01 (0.59)

0.89 (0.48)

–

0.73 (1.36)

zwf

1.20 (0.57)

1.18 (0.49)

–

0.41 (1.38)

gnd

1.05 (0.99)

0.96 (0.79)

–

0.67 (1.07)

zwf and gnd

1.32 (0.98)

1.38 (0.81)

–

0.18 (1.05)

SH9*

–

1.68 (1.68)

0.85 (0.51)

0.78 (1.37)

–

zwf

1.76 (1.75)

0.80 (0.52)

0.87 (1.45)

–

gnd

1.78 (1.64)

0.87 (0.68)

0.71 (1.13)

–

zwf and gnd

1.88 (1.70)

1.40 (0.65)

0.15 (1.28)

–

SH10

zwf and gnd

1.57

1.30

0.38

–

  1. aYields of metabolites were calculated from three individual experiments and the standard deviation was less than 10 %
  2. bRefer to Table 1 for the genotype of the strains
  3. cData for SH8* were obtained from the previous study and presented for comparison [18]
  4. dYields of metabolites in parentheses were obtained with gluconate as carbon source
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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