Table 3 Plasmids constructed and used in this study
Plasmids | Characteristics | Sources |
|---|---|---|
pMD19-T | Delivery vectors | Takara |
pUG6 | Backbone vectors | EUROSCARF |
pSH47 | GAL1 promoter | EUROSCARF |
pSH65 | Cre recombinase gene, Zeocin | EUROSCARF |
pUG6-tmInuB | Ty1-P TDH3 -SP MFα -InuB-T CYC1 | This study |
pUG6-ssInuB | Ty1-P SUC2 -SP SUC2 -InuB-T CYC1 | This study |
pUG6-ssInuC | Ty1-P SUC2 -SP SUC2 -InuC opz -T CYC1 | This study |
pUG6-PT | P TDH3 -T CYC1 | This study |
pUG6-InuMK | P TDH3 -InuMK1-T CYC1 | This study |
pUG6-PEP4 | P GAL1 -PEP4 | This study |
pUG6-HOre | HO cassette | This study |
pUG6-HOdel | Cassette for deletion of the HO gene | This study |
pUC-InuC opz | Codon-optimized InuC gene | This study |