Fig. 2

Screening of effective fatty acyl-CoA reductase (FAR)-encoding genes for fatty alcohol production in Y. lipolytica. All strains were precultured in SD-LEU medium for 24 h and subcultured to SD-LEU (galactose as carbon source for BY4743) in the initial OD₆₀₀ of 0.05. Detection was performed after 24 h subculturing. a Intracellular fatty alcohol amount of Po4f strains expressing FAR gene candidates under the control of TEF promoter. Mafar, Scfar, Atfar1, Atfar6, and mfar1 were obtained through PCR-based cloning, and Mfar1 (codon optimized version of mfar1) and Tafar1 were codon optimized and synthesized. b Comparison of hexadecanol distribution between Y. lipolytica and S. cerevisiae (IN: intracellular, EX: extracellular). Tafar1 was driven by TEF promoter while mfar1 gene was driven by Gal1 promoter. Results are the mean of duplicate experiments and error bars indicate standard deviations