Fig. 3

Effect of deleting potential degradation pathways on fatty alcohol-producing capability. In confirming responsible target genes for improving fatty alcohol production, fatty alcohol-producing capability other than fatty alcohol titer was focused and was represented with intracellular fatty alcohol amount per unit of cells (OD₆₀₀), to eliminate growth retarding effect from gene alternations and intracellularly accumulated fatty alcohol/aldehyde, which can be recovered through further adaptive evolution. Knock-out mutants derived from H222S4 or ura-po4f strains expressing Tafar1 were used for effect evaluation of potential degradation pathways on fatty alcohol-producing capability. After transformation of Tafar1 expression cassette into corresponding strains, transformants were used for inoculation into SD-URA (H222S4-derived strains) or SD-LEU (ura-po4f-derived strains), and fatty alcohol-producing rate was detected after 24 h culturing. Results are the mean of duplicate experiments and error bars indicate standard deviations. H222-S4: ura3, H222ΔP: ura3 pox1-6, H222ΔPΔF: ura3 pox1-6 fao1, H222ΔPΔA: ura3 pox1-6 fadh1 adh1-7